Effects of expressions of NF-?B and survivin on anti-tumor activity of TRAIL in gastric carcinoma cells / 第三军医大学学报

ABSTRACT

Objective To investigate the effects of expressions of NF ?B and survivin on the anti tumor activity of TRAIL in gastric carcinoma cells. Methods Gastric carcinoma cells were cultured in RPMI 1640. The apoptotic rates of gastric carcinoma cells SGC 7901, MKN28, AGS, and MKN45 after treatment with TRAIL were analyzed by flow cytometry. The expressions of NF ?B and survivin in the 4 gastric carcinoma cells were determined by Western blotting. Results At 24 h after gastric carcinoma cells exposed to TRAIL at the dose of 300 ng/mL, the apoptosis percent ages were 36 05% for MKN28, 20 27% for MKN45, 16 50% for AGS, and 11 80% for SGC 7901. Western blotting showed that expressions of NF ?B and survivin were lower in MKN28 than those in MKN45, AGS and SGC 7901. Conclusion The anti tumor sensitivity of TRAIL to gastric carcinoma cells is related to the expressions of NF ?B and survivin.