Regulatory effects of 3 kinds of antifibrotic cytokines on activity of transforming growth factor-?_1 gene promoter / 第二军医大学学报
Academic Journal of Second Military Medical University
;
(12)1999.
Artigo
em Chinês
| WPRIM
| ID: wpr-680302
ABSTRACT
T polymorphism,was selected as putative promoter.The recombinant constructions containing-1328-+812 of TGF-?_1 gene and CAT reporter gene(phTGF2.14T,phTGF2.14C)were constructed and transfected into HepG2 cells with liposomal trans- fection method,then the transfected HepG2 cells were treated with IL-10(4 ng/ml),HGF(10 ng/ml)or IFN-?(20 ng/ml). Reporter gene activity was analyzed by ELISA.Results:
Reporter gene activity in cells transfected with phTGF2.14C was sig- nificantly higher than those transfected with phTGF2.14T(P
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Academic Journal of Second Military Medical University
Ano de publicação:
1999
Tipo de documento:
Artigo
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