LOWER DOSE HELIUM-NEON LASER IRRADIATION PROMOTING NEURONAL GROWTH AND RNA SYNTHESIS IN CULTURE / 解剖学报

ABSTRACT

The superior cervical ganglia (SCG) were dissected from neonatal rats.Dissoci-ated cell cultures were grown in Eagle's MEM supplemented with 20% calf serumwhich contain nerve growth factor (NGF).The cultures were divided into two groups:laser irradiation group and control.The former was exposed to lower dose of Helium-Neon laser (power:4 milliwatt)5 minutes every two hours.Two groups cultured for 20,22,24 and 28 hoursrespectively.Then the length of neurites of neurons were measured.At 22 hours,RNA and DNA synthesis of neurons labeled by ~3H-uridine and ~3H-thymidine wereobserved.The results showed that lower dose of Helium-Neon laser irradiation not onlypromotes growth of neurite but also enhances RNA synthesis of neurons in culture.However DNA synthesis in neurons does not promote in this experiment.