Studies on structural characteristics of protein-bond polysaccharide Le-3 from fruitbody of Lentinus edodos / 中草药

ABSTRACT

Object To study the structural characteristics of protein bond polysaccharide Le 3 from the fruitbody of Lentinus edodos (Berk ) Pegler Methods Relative molecular weights were determined by gel permeation chromatography Structural characteristics were expounded by infra red scan, gas chromatography, ? elimination reaction and Sepharose gel electrophoresis The content of total polysaccharide and protein were determined by colorimetry Results Mean molecular weight and molecular number of Le 3 were 13 700 and 12 500 Typical absorption peak of the polysaccharide as shown in infra red spectrum was of ? type glycosidic linkage Le 3 was composed of arabinose, xylose, mannose, galactose, glucose and glucuronic acid The molar ratio of the neutral saccharides was Ara∶Xyl∶Man∶Gal∶Glu=0 31∶0 47∶1 00∶1 15∶8 92 The content of total saccharide and protein were 70 62% and 25 31% The saccharides were not linked to peptides chain through O glycosidic linkage Le 3 contained RNA Conclusion Le 3 was a kind of new type polysaccharide isolated from the fruitbody of L edodes