HIGH LEVEL EXPRESSION AND ACTIVITY DETECTION OF SINGLE CHAIN IMMUNOTOXIN 183B_2ScFvPE38 AGAINST OVARIAN CARCINOMA / 解剖学报
Acta Anatomica Sinica
; (6)1953.
Article
em Zh
| WPRIM
| ID: wpr-681435
Biblioteca responsável:
WPRO
ABSTRACT
Objective To prepare the immunotoxin protein (183B 2ScFvPE38) which might be useful in immuno guided therapy for ovarian carcinoma and study the activity of the protein. Methods The methods of ELISA and cytotoxicity were used to study the immunotoxin after induced with IPTG and the activity of the immunotoxin. Results The expressed fusion proteins were detected mostly as inclusion bodies at high level, and soluble immunotoxins were also observed. The results showed liable activity of antibody part and toxic part. Conclusion The recombinant fusion protein 183B 2ScFvPE38 keeps the activity of both components and might be of great use in the future to deal with ovarian carcinoma. [
Texto completo:
1
Índice:
WPRIM
Tipo de estudo:
Diagnostic_studies
Idioma:
Zh
Revista:
Acta Anatomica Sinica
Ano de publicação:
1953
Tipo de documento:
Article