Determination of palmatine hydrochloride and berberine hydrochloride in Chinese Mahonia Stem from different habitats by HPLC / 中草药
Chinese Traditional and Herbal Drugs
; (24)1994.
Article
em Zh
| WPRIM
| ID: wpr-681613
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WPRO
ABSTRACT
Object To develop a method for the determination of palmatine hydrochloride and berberine hydrochloride in Chinese Mahonia Stem from different habitats Methods HPLC method was set up, using Intersil ODS 3 C 18 column, the mobile phase was acetontrile water sodium laurylsulfonate (470∶ 530∶1 g), the UV detection wavelength was 265 nm, with a flow rate of 1 0 mL/min at 40 ℃ Results A good linearity was obtained in the range of 4 368 52 416 ?g/mL(r=0 999 9) for palmatine hydrochloride and 4 532 54 384 ?g/mL (r=0 999 9) for berberine hydrochloride The average recovery of palmatine hydrochloride and berberine hydrochloride was 98 97% and 98 98%, respectively Conclusion The method is simple, rapid and with better reproducibility for the determination of palmatine hydrochloride and berberine hydrochloride in Chinese Mahonia Stem
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Índice:
WPRIM
Idioma:
Zh
Revista:
Chinese Traditional and Herbal Drugs
Ano de publicação:
1994
Tipo de documento:
Article