Establishment of method for the analysis of Yun Zhi Polysaccharopeptide(PSP) by HPCE / 中成药

ABSTRACT

Objective: To establish the analytical method for the characteristic electropherograms of PSP by High Performance Capillary Electrophoresis(Capillary Zone Electrophoresis). Method: Types, concentration and pH of electrolyte, voltage, temperature, length and size of column were investigated for the analysis of PSP and BSA which is as standard using capillary zone electrophoresis. Results: The optimum condition is that 10mmol?L -1 boric acid buffer, pH9.18, a column 50?m?57cm(HP, effective length of 48.5cm), 30KV, temperature 25 ?C ,DAD detection. The analysis is ended within 7min. Conclusion: The method is simple, rapid and reliable. The characteristic electropherograms of PSP can be established.