Comparison of Diagnostic Performance of Three Real-Time PCR Kits for Detecting Mycobacterium Species
Yonsei Medical Journal
;
: 301-306, 2011.
Artigo
em Inglês
| WPRIM
| ID: wpr-68176
ABSTRACT
PURPOSE:
PCR is widely used for rapidly and accurately detecting Mycobacterium Species. The purpose of this study was to assess the diagnostic performance of three real-time PCR kits and evaluate the concordance with two older PCR methods. MATERIALS ANDMETHODS:
Using 128 samples, the five PCR methods were assessed, including an in-house PCR protocol, the COBAS Amplicor MTB, the COBAS TaqMan MTB, the AdvanSure TB/NTM real-time PCR, and the Real-Q M. tuberculosis kit. The discrepant results were further examined by DNA sequencing and using the AdvanSure Mycobacteria Genotyping Chip for complete analysis.RESULTS:
For Mycobacterium tuberculosis (MTB) detection, all five kits showed 100% matching results (positive; N = 11 and negative; N = 80). In non-tuberculous mycobacterium (NTM) discrimination, the AdvanSure yielded two true-positive outcomes from M. intracellulare and one false positive outcome, while the Real-Q resulted in one true-positive outcome and one false negative outcome for each case and another false negative result using the provided DNA samples.CONCLUSION:
Real-time PCR, yielded results that were comparable to those of the older PCR methods for detecting MTB. However, there were disagreements among the applied kits in regard to the sample test results for detecting NTM. Therefore, we recommend that additional confirmatory measures such as DNA sequencing should be implemented in such cases, and further research with using a larger numbers of samples is warranted to improve the detection of NTM.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Kit de Reagentes para Diagnóstico
/
Tuberculose
/
DNA Bacteriano
/
Complexo Mycobacterium avium
/
Infecção por Mycobacterium avium-intracellulare
/
Reação em Cadeia da Polimerase
/
Mycobacterium
/
Infecções por Mycobacterium
/
Mycobacterium tuberculosis
Tipo de estudo:
Estudo diagnóstico
/
Guia de Prática Clínica
Limite:
Humanos
Idioma:
Inglês
Revista:
Yonsei Medical Journal
Ano de publicação:
2011
Tipo de documento:
Artigo
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