Determination of emodin and rhein in Niuhuangjiedu Tablets by HPLC / 中成药
Chinese Traditional Patent Medicine
;
(12)1992.
Artigo
em Chinês
| WPRIM
| ID: wpr-682039
ABSTRACT
Objective:
A HPLC method for determination of emodin and rhein in Niuhuangjiedu Tablets was established.Methods:
The chromatographic conditions were listed below Diamonisil ODS C 18 column, UV detector at 254nm of wavelength, a mobile phase of methanol water phosphate acid(720∶280∶1),and 1mL?min -1 of flow rate. The retention time of emodin was 8.49 min and that of rhein was 15.56min.Results:
The calibration curves were linear for emodin within 0.469~1.407?g( r =0.9992) and for rhein within 0.3125~ 0.9375?g ( r = 0.9992). The recoveries were 98.2%( RSD =0.43%)for emodin and 98.4%( RSD =0.72%)for rhein, respectively.Conclusion:
This method is simple and the separate result is satisfactory.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Traditional Patent Medicine
Ano de publicação:
1992
Tipo de documento:
Artigo
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