Study on the mitochondrion DNA mutation in tumor tissues of gynecologic oncology patients / 中华妇产科杂志

ABSTRACT

Objective To investigate the mitochondrion DNA (mtDNA) mutation in tumor tissues of gynecologic oncology patients and their relationship to tumorigenesis and tumor development Methods The samples of tumor tissue and their proximal normal tissue of 32 gynecological malignant tumor patients as well as 8 gynecological benign tumor patients were taken The malignants were 5 squamous cervical carcinomas, 10 endometrial carcinomas and 17 epithelial ovarian cancers(EOC) The benign tumors were 4 ovarian epithelial tumors and 4 uterine myomas Polymerase chain reaction single strain conformation polymorphism(PCR SSCP) and DNA sequencing were done to examine mtDNA mutation Results The mtDNA mutation rate and polymorphism rate were 68 8% and 56 3% respectively in 32 cases of malignants The mtDNA mutation rate and polymorphism rate were 2/8 and 4/8 respectively in 8 cases of benigns The difference between malignants and benigns was significant ( P