The effects of localized thermochemotherapy on angiogenesis and the expression of VEGF protein of C6 gliomas in rat / 中华物理医学与康复杂志

ABSTRACT

Objective To study the effects of localized thermochemotherapy on angiogenesis and the expression of VEGF protein of C6 gliomas in rats,and explore the mechanisms of angiogenesis inhibition by localized thermochemotherapy. Methods Following the establishment of animal models, 40 rats harboring the C6 gliomas were randomly allocated to 4 groups,the rats in the fore 3 groups were treated by localized thermochemotherapy, hyperthermia and chemotherapy, respectively, the remaining group served as control. The expression of VEGF and FⅧ RA protein were detected by S P immunohistochemistry. The vascular structure in rat's glioma was observed by immunohistochemistry and electron microscopy technique. Results As compared with the control group, the expression of VEGF protein was decreased in the hyperthermia and chemotherapy groups,as well as in the thermochemotherapy group.The expression of VEGF was positively correlated with the microvessel density in the tumor ( r =0.9798, P