Study on fingerprints of Redix Paeoniae Rubra by HPLC / 中草药

ABSTRACT

Object To study the HPLC fingerprints and establish a sensitive and specific method for controlling the quality of Radix Paeoniae Rubra (RPR) Methods The HPLC method was applied for quality assessment of RPR All 18 samples collected from different places were determined A Hypersil C 18 column (200 mm?4 6 mm, 5 ?m) was used with the mobile phase being acetonitril 0 025 mol/L H 3PO 4 THF (9 5∶90 5∶1 25), flow rate being 0 8 mL/min, detecting wavelength being 254 nm, the column temperature being at room temperture Results This method had a good repeatability and reproducibility, and the ratio of peaks' area of different habitat samples were different Conclusion The method is suitable to differentiate RPR form different sources conveniently, and can be used as a quality control method for this herb