Preparation and anti-carcinoma effect of magnetic polybutylcyanoacrylate nanosphere loaded with aclacinomycin A / 中华消化杂志

ABSTRACT

Objective To prepare magnetic polybutylcyanoacrylate (PBCA) nanosphere loaded with aclacinomycin A (ACM) and investigate its anti carcinoma effect and toxicity. Methods Magnetic PBCA nanospheres loaded with ACM were prepared by interfacial polymerization method. Female BABL/c nude mice were injected with MKN 45 gastric carcinoma cell line mass subcutaneously near right forefoot to establish human gastric carcinoma model. The mice were divided into 5 groups with 6 mice in each group: ACM (8 mg/kg), nanosphere with low dosage of ACM(1.6 mg/kg), nanosphere with high dosage of ACM (8 mg/kg ), magnetic nanosphere without ACM and normal saline (NS). Magnets (2500 Gs) were embedded in the tumor mass of all the mice one day before the date of therapy. The agents were administered again the same as the first time after 5 days. Tumor weight was measured and assay of colony forming unit granulocyte and macrophage (CFU GM) was performed on semi solid culture. Results The drug content of the agent was 12.0% and the mean particle size was 210 nm. The tumor inhibition rates of ACM, nanosphere with low dosage of ACM, nanosphere with high dosage of ACM and carrier without ACM on human gastric carcinoma in nude mice were 22.63%, 30.66%, 52.55% and 10.22% respectively. The average tumor weight of targeted nanospheres group was much lower than that of the same dosage of non targeted ACM group( P