Determination of chlorogenic acid and hesperidin in Qingrehuoxue Mixture / 中成药

ABSTRACT

AIM:To determine chlorogenic acid and hesperidin in Qingrehuoxue Mixture (Herba Artemisiae Scopariae, Pericarpium Citri Reticulatae, etc.) at the same time by HPLC. METHODS: A gradient method was set up with eluent of acetonitrile-water (0.4% phosphric acid). The detection wavelength of hesperidin was set at 283 nm, the detection wavelength of chlorogenic acid at 325 nm. RESULTS: The average recovery of two components were all above 95%, RSD was no more than 1.6%. The calibration curve was linear. CONCLUSION:The method is accurate and reliable with good reproducibility.