Determinatin of Stachydrine Hydrochloride in Fresh Medicinal Material of Herba Leonuri by HPLC / 中药新药与临床药理
Article
em Zh
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| ID: wpr-682649
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WPRO
ABSTRACT
Objective To establish a HPLC method for the separation and determination of stachydrine hydrochloride in fresh medicinal material of Herba Leonuri.Methods The NH2 column(250 mm? 4.6 mm,5 ? m) was adopted with acetonitrile-water(80 ∶ 20) as mobile phase at a flow rate of 1.0 mL/min.The detection wavelength was at 192 nm.The column temperature was at 22 ℃.The solution for content determination was obtained after extracted with ethanol and then filtered and purified through an activated charcoal-aluminium oxide column with ethanol as eluant.Results Stachydrine hydrochloride in fresh Herba Leonuri can be separated and determined on NH2 column.A good linear range was within 2.29~ 11.46 ? g.The average recovery for stachydrine hydrochloride was 98.26 % and RSD was 1.52 %(n=5).Conclusion The method is steady and with good repeatability,and can be used to determine the content of stachydrine hydrochloride in fresh Herba Leonuri.
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WPRIM
Idioma:
Zh
Revista:
Traditional Chinese Drug Research & Clinical Pharmacology
Ano de publicação:
1993
Tipo de documento:
Article