Effects of estrogen receptor ? on proliferation of ovarian clear cell adenocarcinoma ES-2 in vitro and in vivo / 中华妇产科杂志

ABSTRACT

Objective To investigate the involvement of estrogen receptor(ER)? in the proliferation of ovarian clear cell adenocarcinoma by restoring ER? expression in a cell line ES-2.Methods A plasmid with full length ER? cDNA,pRSV-ER? and its negative vector control pRSV were introduced into ES-2.The cells transfected were named according to the plasmids:ES-pRSV,ES-pRSV-ER?.RT-PCR and western blot were used to detect the expression of ER? in ES-2,ES-pRSV and ES-pRSV-ER? cells.The growth activities of cells in vitro were detected by methyl thiazolyl tetrazolium(MTT)assay,and in vivo growth in nude mice was also observed.Flow cytometry was performed to show the change of cell cycles. Results The ES-pRSV-ER? cells were identified with ER? mRNA and protein expression.The growth activities of ES-pRSV-ER? were inhibited in vitro.In MTT analysis,the values of ES-2,ES-pRSV,and ES- pRSV-ER? cells were 0.78?0.05,0.81?0.06,and 0.53?0.07(the third was lower compared with the former two,P