Frequency and significance of CD4~+CD25~+FOXP3~+T regulator cells in the peripheral blood of systemic lupus erythematosus patients / 中华风湿病学杂志

ABSTRACT

Objective To investigate the frequency of CD4~+CD25~+FOXP3~+Treg cells in the peripheral blood of systemic lupus erythematosus(SLE)patients and its association with disease activity.Methods Pe- ripheral blood mononuclear cells(PBMC)from 28 patients(including 18 active SLE)and 22 healthy controls were counted and stained for CD4,CD25 and intracellular FOXP3.Cells were examined by 3-color staining on the Epics XL-MC and data were analyzed using EXPO32 software.Disease activity was assessed by systemic lupus erythematousus activity index(SLEDAI).Results The frequency of CD4~+CD25~+FOXP3~+Treg cells was significantly decreased in patients with active SLE compared with patients with inactive SEE and controls [(1.08?0.43)%,(1.58?0.45)% and(1.66?0.34)%,P