Direct gene transfer of nacked DNA encoding vascular endothelial growth factor into ischemic swine myocardium / 中国介入心脏病学杂志

ABSTRACT

Objective To investigate the efficacy of vascular endothelial growth factor (VEGF) gene therapy in coronary occlusion of animal Methods 19 swine underwent left thoracotomy followed by the ligation of left anterior descending coronary artery Constructed pcD 2/hVEGF 121 eukaryotic expression plasmid was directly injected into the swine myocardium RT PCR, immunohistochemistry of VEGF and factor Ⅷ related antigen were used to detect VEGF gene expression and biological effect Coronary angiography was done to evaluate collateral circulation of the occluded artery Results High levels of VEGF mRNA and protein expression were detected There was a significant increase in the number of capillaries when compared with control group Coronary angiography showed better collateral circulation in VEGF group Conclusion Direct injection of pcD 2/hVEGF 121 eukaryotic expression plasmid can transfect the myocardium and express VEGF protein This gene product can increase capillary number and enhance collateral circulation of the occluded coronary