The Effect of Sepia on the Activity of TPK,PKC and PKA in H_(22) Cancer Cells / 中国海洋药物

ABSTRACT

The effect of Sepia on the activity of tyrosine protein kinase (TPK) protein kinase C (PKC) and cAMP-dependent protein kinase (PKA) was studied in H 22 cancer cells by using the incorporation of ? 32 P ATP into exogenous substract.The results showed that membranous TPK activity of membrane,cytosol and mitochondria was elevated significantly,while the membranous TPK activity was increased obviously;while the cytosolic PKC activity was increased most.The cytosolic PKA activity was increased.These results suggested that Sepia can changeover the activity of TPK,PKC and PKA,which was abnormally changed by cancer cells,then caused that the up regulation was diminished and the down regulation was enhanced towards the Ras MAPK signal transduction pathways.This inhibited or interrupted the cascade transduction of the Ras MAPK pathway,thus produced the effect of anticancer and accelerated the differentiation by transforming cancer cells from undifferentiated proliferation to differentiated proliferation.