Molecular Variation Based on mtDNA-COII Gene of Members of the Anopheles minimus Group / 中国寄生虫学与寄生虫病杂志

ABSTRACT

Objective To investigate intra and inter species molecular variations and phylogeny of the five members of the Minimus Group of Anopheles subgenus Cellia in China An. aconitus, An.varuna, An.jeyporiensis, An.minimus A and An.minimus C, and to report the DNA sequence divergence among these species at a mitochondrial locus (cytochrome oxidase II). Methods Single mosquito′s legs were digested to extract DNA. COII gene was amplified, sequenced and analysed; and the phylogenetic tree of members of An.minimus group was reconstructed by maximum likelihood method (DNAML). Results and Conclusion The data confirmed the presence of two cryptic species, A and C, within An.minimus complex in the research localities; and two species differed by 2.3% in the COII sequences owing to 16 nucleotide substitutions. There was less variation between the two species than other members of the Minimus Group.