Regulation of expression of TGF-?_1 and TGF -?type Ⅱ receptor in kidney of diabetic rat treated by losartan / 中国糖尿病杂志

ABSTRACT

ObjectiveTo investigate the kidney protective e ffects of losartan in diabetic rat and its kidney protective mechanism. MethodsThe rats were randomly divided into the following groupsnorm al control (group C), streptozotocin diabetic (group DM) and diabetic rats t reated with losartan (group DL). After eight weeks study, the mRNA expressions of transforming growth factor ? 1(TGF ? 1), TGF ?type Ⅱ receptor (T? RⅡ)and fibronectin in renal cortices of all three groups were measured by quan titative reverse transcription and polymerase chain reaction(RT PCR). The prote in expressions of transforming growth factor ? 1(TGF ? 1), T?RⅡ and fib ronectin in renal cortices of all three groups were measured by histoimmunochemi stry.The levels of blood glucose,urea and creatinine were measured by biochemic al assy.The concentrations of blood insulin and angiotensin Ⅱ were measured by radioimmunoassay. The rates of urinary albumin excretion were measured by sulfos alicylic acid assay.ResultsAfter eight weeks, mean glomerula r volume, kidney weight / body weight ratio, urinary albumin excretion ratio, bl ood urea and creatinine were significantly higher in diabetic group than in norm al control group(P