Analysis of the Sequences of the Ribosomal DNA ITS_2 Region for Differenciating Anopheline Mosquitoes within Hyrcanus Group / 中国寄生虫学与寄生虫病杂志
Chinese Journal of Parasitology and Parasitic Diseases
;
(6)1997.
Artigo
em Chinês
| WPRIM
| ID: wpr-684595
ABSTRACT
Objective To analyze the genetic characteristics of ribosomal DNA ITS 2 region for differenciating anopheline mosquitoes within Hyrcanus group. Methods The ribosomal DNA ITS 2 region of both laboratory line and filed collected An. anthropophagus and An. sinensis as well as the field collected An. yatsushiroensis and An. lester i were amplified and sequenced. The sequencing data were then analyzed for the restriction mapping using Omega Sequencing analysis program. Results and Conclusion The length of the sequences of An. sinensis, An. anthropophagus, An. yatsushiroensis and An. lesteri are 472, 452, 456 and 456 bp respectively. The restriction mapping showed that there were different restriction digesting sites among the ribosomal DNA ITS2 region sequences from An. sinensis, An. anthropophagus, An. yatsushiroensis and An. lesteri . On the basis of the sequence differences among the anopheline species within Hyrcanus group, it is possible to develop new technique for genetic identification of anopheline mosquitoes.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Tipo de estudo:
Estudo prognóstico
Idioma:
Chinês
Revista:
Chinese Journal of Parasitology and Parasitic Diseases
Ano de publicação:
1997
Tipo de documento:
Artigo
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