Application of two bacteria colony-PCR methods in the screening of phage antibody library / 医学研究生学报
Journal of Medical Postgraduates
;
(12)2003.
Artigo
em Chinês
| WPRIM
| ID: wpr-684635
ABSTRACT
Objective:
To value the application of two bacteria colony-PCR methods in the screening of phage antibody library.Methods:
Five positive monoclonal bacterium were respectively suspended in either deionized water or 0.1% Triton X-100, and then boiled to be used as template in PCR. . The DNAs products of PCR were extracted and digested by two enzymes, and then determined by electrophoresis.Results:
The inserted genes were detected in all the 5 clones after PCR and enzyme digestion .Conclusion:
Bacteria colony-PCR can be used in screening positive recombinant colonies. The bacteria colony-PCR method with bacteria colonies suspended in deionized water is valuable in large scale positive recombinant bacterium screening.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Tipo de estudo:
Estudo diagnóstico
/
Estudo de rastreamento
Idioma:
Chinês
Revista:
Journal of Medical Postgraduates
Ano de publicação:
2003
Tipo de documento:
Artigo
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