Heterogenous Gene Expression of Methyl Parathion Hydrolase and Analysis of the Enzyme Activity / 微生物学通报
Microbiology
;
(12)1992.
Artigo
em Chinês
| WPRIM
| ID: wpr-684642
ABSTRACT
Methyl parathion hydrolase (MPH, E.C.3.1.8.1) coding gene mph from Pseudomonas sp. WBC-3, isolated and identified by our lab, was successfully expressed in E. coli AD494 (DE3)/ pET32a(+) system as soluble fusion form at high level. The recombinant MPH showed nearly 4~5 fold higher specific activity to parathion than the enzyme from Pseudomonas sp. WBC-3. In addition, the thermal stability of the recombinant enzyme was improved comparing with the wild type enzyme.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Microbiology
Ano de publicação:
1992
Tipo de documento:
Artigo
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