Analysis of Sponge-associated Culturable and Natural Microbial Community Structure Based on DGGE Fingerprinting / 微生物学通报

ABSTRACT

The microbial community structure of 4 kinds of sponges in South China Sea were revealed using culture-independent PCR- DGGE gene fingerprint technique as well as the mixed culturable microorganism. The relationship between the culturable and un-culturable microorganism was studied based on the different fingerprint profile. It was found that there is host specificity for microorganism in different sponges from the same marine area. Culture condition such as medium is an important factor for sponge-associated microbial cultivation and the culturable microorganism amount to only a little part of the total sponge-associated microorganisms.