Expressing, Purification and Identification of Neuritin Gene in the E. coli / 中国生物工程杂志
China Biotechnology
; (12)2006.
Article
em Zh
| WPRIM
| ID: wpr-684933
Biblioteca responsável:
WPRO
ABSTRACT
Neuritin is a new neurotrophic factor found recently. In order to identify the function of Neuritin clearly, the coding sequence of human neuritin was amplified by PCR from neuritin cDNA , this fragment digested by NocI and NotI was inserted into pET32a by T4 ligase and transformed into E. coli BL21 then the recombinant plasmid named pET32a-neuritin was constructed successfully . Neuritin was expressed distinctly after inducing by EPTG. The product was identified as neuritin by SDS-PAGE and Western blot analysis . The expression production was purified on Ni2+-NTA column.
Texto completo:
1
Índice:
WPRIM
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Idioma:
Zh
Revista:
China Biotechnology
Ano de publicação:
2006
Tipo de documento:
Article