Disruption of hom Gene Encoding for Homoserine Dehydrogenase of Corynebacterium glutamicum / 中国生物工程杂志

ABSTRACT

The hom gene encoding for homoserine dehydrogenase was amplified from the genomic DNA of Corynebacterium glutamicum ATCC 13032.After the kanamycin-resistant gene(Km)cassette from plasmid pET28a was inserted into the center of hom,the homKm cassette was then electroporated into the competent cell of C.glutamicum ATCC 13032.And kanamycin-resistant clones were obtained.PCR was performed to confirm whether the Km gene was integrated into the hom gene of these clones and the recombinant strains of hom-disrupted were screened out.Fermentation results showed that the lysine yield of the hom-disrupted strain C.g-homKm-8 reached 4.7 g/L,which was 6.7 times that of C.glutamicum ATCC 13032.