System of Intein-mediated PHB Purified Human Antimicrobial Peptide LL-37 / 中国生物工程杂志
China Biotechnology
; (12)2006.
Article
em Zh
| WPRIM
| ID: wpr-686100
Biblioteca responsável:
WPRO
ABSTRACT
The new intein-mediated PHB purify protein system is a high expression, automatic cutting, for purification, low-cost protein purification system,it is conducive to large-scale protein purification.Choose human antibacterial peptide LL-37 as the purification objects,which is poison to prokaryotic cell.We construct intein-mediated PHB purified human antimicrobial peptide LL-37 system through genetic engineering technology and use this system to purify LL-37. The results show that this system can highly express LL-37 fusion protein and purifiy the product as same size with expectations.
Texto completo:
1
Índice:
WPRIM
Idioma:
Zh
Revista:
China Biotechnology
Ano de publicação:
2006
Tipo de documento:
Article