Comparison between Human Follicular Fluid and SpermGrad for Sperm Preparation in Asthenozoospermia / 대한불임학회지
Korean Journal of Fertility and Sterility
;
: 53-60, 2006.
Artigo
em Coreano
| WPRIM
| ID: wpr-68613
ABSTRACT
OBJECTIVE:
We tested the usefulness of swim-down technique using human follicular fluid (hFF) in sperm preparation.METHODS:
Semen samples were obtained from twelve male partners showing asthenozoospermia (sperm motility < 50%) at the time of routine andrologic evaluation in Seoul National University Bundang Hospital. After dividing into two aliquots, each samples were processed either by swim-down using 100% hFF or density gradient using SpermGrad. Sperm quality was assessed by computer-assisted semen analyzer (CASA).RESULTS:
Motility, Rapid motility, VCL (curvilinear velocity), ALH (amplitude of lateral head displacement), and hyperactivated sperms were significantly increased, and LIN (mean linearity) was decreased significantly after sperm preparation in both groups. Motility was significantly higher after swim-down using 100% hFF when compared with density gradient using SpermGrad (81.2+/-4.7 vs. 67.6+/-2.3, p=0.02). The other parameters assessed by CASA were not different between the two methods.CONCLUSION:
Swim-down method with 100% hFF may be a useful method in preparation of sperm from asthenozoospermia.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Sêmen
/
Espermatozoides
/
Líquido Folicular
/
Astenozoospermia
/
Seul
/
Cabeça
Limite:
Feminino
/
Humanos
/
Masculino
País/Região como assunto:
Ásia
Idioma:
Coreano
Revista:
Korean Journal of Fertility and Sterility
Ano de publicação:
2006
Tipo de documento:
Artigo
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