Control Mechanisms of Ovarian Follicle Development by Follicle Stimulating Hormone and Pituitary Adenylate Cyclase-activating Polypeptide / 대한불임학회지
Korean Journal of Fertility and Sterility
;
: 15-24, 2006.
Artigo
em Inglês
| WPRIM
| ID: wpr-68617
ABSTRACT
OBJECTIVE:
Pituitary adenylate cyclase-activating polypeptide (PACAP), a novel hypothalamic neuropeptide, has been suggested to play a role in ovarian folliculogenesis. The present study evaluated the effect of PACAP on the growth of preantral follicles.METHODS:
Preantral follicles were mechanically isolated from ovaries of 21-day-old rats and cultured in groups for 3 days in serum-free medium in the absence or presence of PACAP-38 (10-6 M).RESULTS:
Treatment with PACAP-38 resulted in an increase in follicle diameter by 75% whereas treatment with follicle stimulating hormone (FSH) increased follicle diameter by 65%. PACAP-38 treatment enhanced the granulosa cell proliferation as measured by thymidine incorporation analysis. Furthermore, the production of progesterone by cultured granulosa cells and GFSHR-17 cell line was stimulated by PACAP-38. Interestingly, PACAP enhanced FSH action on stimulation of SF-1 and aromatase gene expression.CONCLUSION:
The present results demonstrate that PACAP stimulated preantral follicle growth by potentiating proliferation and by stimulating steroidogenesis.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Ovário
/
Progesterona
/
Timidina
/
Neuropeptídeos
/
Aromatase
/
Expressão Gênica
/
Linhagem Celular
/
Polipeptídeo Hipofisário Ativador de Adenilato Ciclase
/
Hormônio Foliculoestimulante
/
Folículo Ovariano
Limite:
Animais
Idioma:
Inglês
Revista:
Korean Journal of Fertility and Sterility
Ano de publicação:
2006
Tipo de documento:
Artigo
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