Development of a Reverse Dot Blot System for Rapid Detection of HBV Genotypes / 中国生物工程杂志
China Biotechnology
;
(12)2006.
Artigo
em Chinês
| WPRIM
| ID: wpr-686423
ABSTRACT
To develop a reverse dot blot assay for rapid detection of HBV genotypes.Specific oligonucleotides probes were desighed and immobilized on nylon membranes.The DNA sample to be tested was PCR-amplified with DIG labeling primers and then hybridized with the immobilized probes.This procedure for detecting HBV genotypes was simple,rapid and specificity.30 specimens in Chongqing area were collected and detected by this method,and results were evaluated using direct sequencing.Results showed that This new method was applicable to precise detection HBV genotypes for specimen with copies up to 103,and the HBV genotyping results showed that genotype B was the predominant genotype in Chongqing area.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Tipo de estudo:
Estudo diagnóstico
Idioma:
Chinês
Revista:
China Biotechnology
Ano de publicação:
2006
Tipo de documento:
Artigo
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