Nucleic acid detection technology used in blood screening of blood donors / 国际检验医学杂志

ABSTRACT

Objective To evaluate the necessity and feasibility of nucleic acid test for donors blood screening .Methods From July 1 ,2011 to December 31 ,2014 ,a total of 170 316 blood samples which were negative in enzyme-linked immunosorbent assay (ELISA)and qualified in aianine aminotransferase detection ,were selected in this study stochastically .All the samples were detected hepatitis B virus(HBV) ,hepatitis C virus(HCV) ,human immunodeficiency virus(HIV) by nucleic acid amplification technology (NAT) .NAT positive samples were reconfirmed in National Center for Clinical Laboratories(NCCL) .Results A total of 160 cases of nucleic acid reactive samples were found out ,the total response rate was 0 .09% ,The response rate of Roche nucleic acid detec-tion system was 0 .10% ,response rate of David nucleic acid detection system was 0 .08% ,there was no significant difference be-tween the two methods(P>0 .05) .In 27 cases of specimens ,14 cases were confirmed as HBV DNA positive ,no HCV RNA and HIV RNA were detected ,the confirmed positive rate was 51 .85% .There were 2 samples detected by chemiluminescence HBsAg reactivity .Conclusion ELISA screening of blood donors has missing phenomenon ,nucleic acid detection method could be used as an effective supplement of the ELISA ,could improve the safety of blood for clinical use ,detection sensitivity is better than ELISA .