Study on correlation between rs4731702 polymorphism of KLF14 gene and gestational diabetes mellitus* / 国际检验医学杂志

ABSTRACT

Objective To investigate the correlation between KLF14 gene rs4731702 locus polymorphism and gestational diabe-tes mellitus (GDM) and relation between its different genotypes with BMI and insulin resistance.Methods This study adopted the case-control method.One hundred pregnant women of GDM (GDM group) and one hundred healthy pregnant women (normal con-trol group) were randomly selected as the research subjects and performed the physical examination ,biochemical indicators detec-tion.HOMA-IR and HOMA-β were calculated.The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was adopted.The genotyping of KLF14 gene rs4731702 locus in the two groups was performed.The genotypes and allele gene frequency were compared between the two groups and the GDM association analysis was conducted.Results The C alleles fre-quency and CC genotype frequency of KLF14 gene rs4731702 locus in the GDM group was significantly higher than that in the con-trol group ,the difference was statistically significant (P< 0.05).The patients with genotype CC in the GDM group had higher BMI ,FPG ,TG ,HbA1c and HOMA-IR as compared with those carrying genotype CT and TT ,the difference was statistically signif-icant(P<0.05).Also they had lower FINS ,HDL and HOMA-βas compared with carriers of genotype CT and TT ,the difference was statistically significant(P<0.05).The multivariate analysis showed that the genotype CC of KLF14 gene rs4731702 locus was closely related with GDM (P=0.005 ,RR=25.128).Conclusion The genotype CC of KLF14 gene rs4731702 locus plays a role in the pathogenesis process of GDM ,may be susceptibility genes for GDM ,which is also related to the abnormal lipid metabolism ,islet dysfunction and obesity.The polymorphism study of KLF14 gene rs4731702 locus helps to reveal the relation between lipid metabo-lism abnormality and insulin resistance with GDM onset.