Optimal Concentration of hEGF for Epithelial Healing in Experimental Corneal Alkali Wounds
Journal of the Korean Ophthalmological Society
;
: 1771-1777, 2001.
Artigo
em Coreano
| WPRIM
| ID: wpr-68826
ABSTRACT
PURPOSE:
An optimal concentration of hEGF to enhance epithelial healing was determined in the rabbit eyes after alkali wounds.METHODS:
Alkali-injured corneas (=5.5 mm, 1N NaOH, 60s) were treated with 0 (control), 2, 5, 10, and 50microgram/ml hEGF 3 times per day. The measurement of epithelial healing rate, transmission electron microscopy and immunohistochemical observation were performed after 7 days treatment.RESULTS:
No significant difference was observed in the rates of epithelial healing between control and each hEGF-treated group. Among the tested concentrations, 5microgram/ml hEGF induced the most active proliferation of basal cells and 50microgram/ml hEGF remarkably produced a vascular ingrowth to the central wound area. The number of epithelial layers was increased by hEGF in a concentration-dependent manner.CONCLUSIONS:
A low concentration of hEGF may selectively enhance epithelial healing without inducing corneal neovascularization. The optimal concentration of hEGF for the stimulation of basal cell proliferation appears to be 5 microgram/ml in rabbit corneal alkali wounds.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Ferimentos e Lesões
/
Neovascularização da Córnea
/
Córnea
/
Microscopia Eletrônica de Transmissão
/
Proliferação de Células
/
Álcalis
Idioma:
Coreano
Revista:
Journal of the Korean Ophthalmological Society
Ano de publicação:
2001
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS