Detection of HHV-8 Virus in 450 Free Blood Donons in Wuhan and Its Genotyping Based on ORFK1 Gene / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 1198-1203, 2018.
Artigo
em Chinês
| WPRIM
| ID: wpr-689506
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the infection of HHV-8 virus in free blood donors in Wuhan.</p><p><b>METHODS</b>Whole blood samples were collected from 450 free blood donors in Wuhan, and the genomic DNA extraction and serum collection were performed respectively. And then, the positive rate of HHV-8 was detected by PCR and ELISA, the positive detection rates were compared between them. Finally, HHV-8 ORFK1 gene was cloned by PCR in the positive samples, and the HHV-8 ORFK1 gene th was genetyped through sequencing analysis, homology comparison and phylogenetic tree construction.</p><p><b>RESULTS</b>25 and 23 cases of positive samples were detected by PCR and ELISA, their positive rate were 5.56% and 5.11% respectively, and without statistically significant difference using χ test analysis (P > 0.05). Based on the results of ORFK1 gene cloning and sequence analysis in 23 positive samples, 15 samples C subtype of had HHV-8 ORFK1 gene, and 8 cases had A subtype had HHV-8 ORFK1 gene.</p><p><b>CONCLUSION</b>There is a certain percentage of HHV-8 infection in the free blood donors in Wuhan. It is suggested to increase the HHV-8 virus screening for free blood donors, so as to ensure the quality of blood.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Filogenia
/
Doadores de Sangue
/
DNA Viral
/
Reação em Cadeia da Polimerase
/
Herpesvirus Humano 8
/
Genótipo
Tipo de estudo:
Estudo diagnóstico
Idioma:
Chinês
Revista:
Journal of Experimental Hematology
Ano de publicação:
2018
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS