Artesunate Suppresses Cell Proliferation of THP-1 Cells by Inducing Apoptosis / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 716-721, 2018.
Artigo
em Chinês
| WPRIM
| ID: wpr-689587
ABSTRACT
<p><b>OBJECTIVE</b>To investigate whether Artesunate(ART) can inhibit the proliferation of THP-1 cells and to explore the potential mechanism of its anti-leukemia effect.</p><p><b>METHODS</b>THP-1 cells were treated with 5 concentrations of Artesunate for 24 h, 48 h or 72 h. The viability of cells was detected with CCK-8 assay, apoptosis was assessed by using flow cytometry, and the STAT3, Caspase3 and Caspase8 protein levels were measured with Western blot .</p><p><b>RESULTS</b>Compared with the control group, ART significantly inhibited the proliferation of THP-1 cells in a dose-dependent manner (r=0.9829, P<0.05). ART also increased the apoptosis of THP-1 cells. The results of Western blot showed that after treated with ART, the STAT3 protein expression in THP-1 cells was significantly down-regulated (P<0.01), and the expressions of Caspase3, cleaved Caspase3 and Caspase8 proteins were up-regulated(P<0.01).</p><p><b>CONCLUSION</b>Artesunate can inhibit the proliferation of THP-1 cells, which may relate with the down-regulation of STAT3 expression and the activation of Capase3 and Caspase8.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Apoptose
/
Artemisininas
/
Linhagem Celular Tumoral
/
Proliferação de Células
/
Células THP-1
/
Artesunato
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Experimental Hematology
Ano de publicação:
2018
Tipo de documento:
Artigo
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