The subsequent biological effects of simulated microgravity on endothelial cell growth in HUVECs / 中华创伤杂志(英文版)
Chinese Journal of Traumatology
; (6): 229-237, 2018.
Article
em En
| WPRIM
| ID: wpr-691006
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>PURPOSE</b>Microgravity is known to cause endothelium dysfunction in astronauts returning from spaceflight. We aimed to reveal the regulatory mechanism in alterations of human endothelial cells after simulated microgravity (SMG).</p><p><b>METHODS</b>We utilized the rotary cell culture system (RCCS-1) to explore the subsequent effects of SMG on human umbilical vein endothelial cells (HUVECs).</p><p><b>RESULTS</b>SMG-treated HUVECs appeared obvious growth inhibition after return to normal gravity, which might be attributed to a set of responses including alteration of cytoskeleton, decreased cell adhesion capacity and increased apoptosis. Expression levels of mTOR and its downstream Apaf-1 were increased during subsequent culturing after SMG. miR-22 was up-regulated and its target genes SRF and LAMC1 were down-regulated at mRNA levels. LAMC1 siRNAs reduced cell adhesion rate and inhibited stress fiber formation while SRF siRNAs caused apoptosis.</p><p><b>CONCLUSION</b>SMG has the subsequent biological effects on HUVECs, resulting in growth inhibition through mTOR signaling and miR-22-mediated mechanism.</p>
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Texto completo:
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Índice:
WPRIM
Assunto principal:
Fisiologia
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Células Cultivadas
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Laminina
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Apoptose
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Simulação de Ausência de Peso
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MicroRNAs
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Proliferação de Células
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Células Endoteliais da Veia Umbilical Humana
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Genética
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
Chinese Journal of Traumatology
Ano de publicação:
2018
Tipo de documento:
Article