The study of clinical indicators for identification of colonization and infection of Acinetobacter baumannii / 安徽医科大学学报

ABSTRACT

Objective To explore the clinical significance of T lymphocyte subsets and cytokines in the identifica-tion of Acinetobacter baumannii colonization and infection. Methods A total of 109 patients in hospital who had A. baumannii in sputum were collected. All patients were divided into colonization group (53 cases) and infection group (56 cases) according to the diagnostic criteria of hospital-acquired pneumonia by respiratory society, Chinese medical association. Another 50 healthy cases as control group. T lymphocyte subgroup and cytokines in peripheral blood were measured, the differences of T lymphocyte subgroup or cytokines between the groups were also com-pared. The factors of T lymphocyte subgroup or cytokines were analyzed by Spearman correlation analysis. Logistic regression analysis was used for investigating the predictors for the infection of A. baumannii. Results Between colonization group and infection group, the significant differences were been found in T lymphocytes subgroup, TNF-α,IFN-γ and IL-17. The A. baumannii infection was positively associated with TNF-α ( rs=0.241, P =0.012), IFN-γ (rs=0.235, P=0.014), IL-2(rs=0.249, P=0.009), IL-4(rs=0.268, P=0.005) and IL-17 (rs=0.538, P=0.000), whereas which was negatively associated with CD3 +(rs= -0.193, P=0.045) and CD4 +/CD8 +(rs= -0.302, P=0.001). IFN-γ and IL-17 was the independent factors for discrimination of A. baumannii infection by multivariate Logistic regression analysis. Conclusion Serum IFN-γ, IL-17 can be used as an indicator of Acinetobacter baumannii infection and colonization, and can provide a basis for the rational use of antimicrobial agents and nosocomial infection prevention and control.