Preparation of icariin/gelatin nanocomposite-PLGA sustained release system and its optimization of technology / 吉林大学学报(医学版)
Journal of Jilin University(Medicine Edition)
;
(6): 438-443, 2018.
Artigo
em Chinês
| WPRIM
| ID: wpr-691594
ABSTRACT
Objective:
To prepare the sustained release system of icariin(ICA)@ gelatin nanoparticles (GNPs)-polyactic-co-glycolic acid(PLGA)(ICA @ GNPs-PLGA), and to optimize the conditions.Methods:
ICA@GNPs-PLGA sustained release system was prepared using two-step desolvation method and S/O/W emulsion solvent-evaporation technique.The effects of different conditions,such as the PLGAGNPs mass ratio and the total quality of ICA added on the entrapment efficiency(EE)of ICA@GNPs-PLGA composite microspheres were detected to optimize the preparation process. The surface morphology of GNPs and ICA@GNPs-PLGA composite microspheres were observed by SEM.The EE and the release results of ICA@GNPs-PLGA in the sample were determined with HPLC.Results:
The prepared composite microspheres and nanocomplex were were white powder.The SEM results showed that the composite microspheres and nanocomplexs were spherical,the surfaces were smoothy,and the particle size distribution range was 4-12 μm and 150-200 nm,respectively,relatively uniform.At a GNPs mass fraction of 6 mg,the critical concentration of PLGA in DCM ranged within 0.5%-1.0%.At a GNPs mass fraction of 12 mg,the critical concentration of PLGA in DCM ranged within 1.0%-2.0%.However,at a critical PLGA mass fraction lower than 0.25%,no fully formed composite microspheres were observed.Within the critical concentration,the average EE of ICA@GNPs-PLGA microspheres was higher than(62.00±1.25)%.In addition,the EE of ICA in the microspheres was negatively correlated with the quality of ICA added.The accumulative release rate was less than in 24 h and it was 65.21% in 40 d.Conclusion:
The ICA@GNPs-PLGA microspheres with homogeneous particle size distribution,high EE,low initial burst and without agglomeration can be acquired under the optimized conditions.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Journal of Jilin University(Medicine Edition)
Ano de publicação:
2018
Tipo de documento:
Artigo
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