Correlation of serum level of TNF-α and TGF-β1 with infiltration degree of lymphocytes in labial glands and interstitial lung disease in patients with primary Sjogren's syndrome / 重庆医学

ABSTRACT

Objective To investigate the correlation between peripheral serum TNF-α and TGF-β1 expression levels with the infiltration degree of lymphocytes in labial glands and interstitial lung disease(ILD) in the patients with primary Sjogren's syndrome (pSS).Methods Serum level of TNF-α and TGF-β1 in 116 patients with pSS and 20 persons undergoing physical examination were measured by ELISA.Meanwhile the labial gland samples in pSS patients were performed the pathological examination.The patients were grouped according to the lesion stage of ILD and pathology grade of labial glands.The serum TNF-α and TGF-β1 levels were statistically analyzed.Results Serum levels of TNF-α and TGF-β1 in the pSS-ILD group were significantly higher than those in the simple pSS group and control group(P＜0.01).The levels of serum TNF-α and TGF-β1 in the simple pSS group were significantly higher than those in the control group(P＜0.01).The levels of serum TNF-α and TGF-β1 in the labial glands lymphocytes infiltration group were significantly higher than those in the labial glands lymphocytes non-infiltration group and control group(P＜0.01),while the levels of serum TNF-α and TGF-β1 in the labial glands lymphocytes non-infiltration group were significantly higher than those in the control group(P＜0.01).The TNF-α and TGF-β1 expression was positively correlated with lymphocytes infiltration lesion grade(r=0.867,0.613,P=0.000);the expression of TNF-α and TGF-β1 was positively correlated with the lesion grade of ILD(r=0.814,0.864,P =0.000);the level of TNF-α was positively correlated with the TGF-β1 relative expression amount(r=0.857,P=0.000).Conclusion TNF-α and TGF-β1 are involved in the lymphocytes infiltration of labial glands and ILD occurrence and develvp ment,and their expression increase may be the cause of labial glands structure destruction and complicating ILD.