Effect of macrophage FRβ expression on bleomycin induced pulmonary fibrosis in mice / 重庆医学

ABSTRACT

Objective To investigate the effect of macrophage FRβ expression on bleomycin induced pulmonary fibrosis(PF) in mice.Methods The rats were divided into the normal group,control group and experimental group,6 cases in each group.The mice were performed the PF induction.The experimental group was treated with immunotoxin,the control group was given the contrast protein and the normal group was not treated.The mouse left lung was used for histological analysis,and the right lung was used for hydroxyproline analysi s.The effect of macrophage FRβ expression on bleomycin induced pulmonary fibrosis in mice and the pulmonary macrophage FRβ expression in the patients with idiopathic pulmonary fibrosis(UIP) were detected.Results The macrophage FRβ expression mainly existed in the patients with UIP and pulmonary fibrosis area of PF mice induced by bleomycin;the survival rate was significantly increased by giving the mice immunotoxin with nose(P=0.003),and the level of total hydroxyproline and fibrosis of PF mice induced by bleomycin was decreased(P=0.009,0.014);immunohistochemistry results showed that immunotoxin could reduce the cells number of lung tumor necrosis factor(TNF)-α,chemotactic CCL2 and CCL12 cells inPF mice induced by bleomycin(P=0.000).Conclusion The FRβ expression of macrophages plays a pathogenic role in IPF,and the targeted therapy of FRβ expression in macrophages may be an effective method for the treatment of IPF.