The effect and underline mechanism of SMC4 on the proliferation, migration and invasion of human breast cancer cell MDA-MB-231 / 重庆医学

ABSTRACT

Objective To explore the effect of down-regulation of structural maintenance of chromosome 4 (SMC4) on proliferation,migration and invasion capability of human breast cancer cell line MDA-MB-231 and its possible mechanism.Methods The expressions of SMC4 in breast cancer tissues and the corresponding adjacent tissues from 20 patients with breast cancer were detected by qPCR.The expressions of SMC4 in human mammary epithelial cell line (MCF10A) and breast cancer cell lines (MDA-MB-231,T47D,SK-BR-3,MCF7 and MDA-MB-468) were detected by qPCR and western blot.After down-regulated the expression of SMC4 in MDA-MB-231 by small interfering RNA (siRNA),qPCR and western blot were used to determine the effect of transfection,CCK8 and clone formation assay were used to detect the proliferation and clonogenicity,Transwell chamber assay was used to detect the migration and invasion,and the possible pathway associated proteins were detected by western blot.Results The expression level of SMC4 in breast cancer tissues was higher than that in corresponding adjacent tissues (P＜0.05).The expression levels of SMC4 in breast cancer cell lines (MDA-MB-231,T47D,SK-BR-3,MCF7 and MDA-MB-468) were higher than that in MCF10A (P＜0.05).After successfully down-regulated SMC4 expression by siRNA,the proliferation,migration and invasion capability of MDA-MB-231 were significantly decreased (P＜0.05),and the expressions of pAKT and p-PI3K were significantly decreased (P＜0.05),whereas theexpressions of AKT and PI3K were not significantly affected.Conclusion Down-regulating the expression of SMCA can inhibit proliferation,migration and invasion capability of MDA-MP-231,which may be related to the activation of PI3K/AKT signaling pathway.