Study on expression and mutual regulation of serum IL-35 and miR-21 in septic patients / 国际检验医学杂志

ABSTRACT

Objective To detect the expression level and correlation of interleukin 35(IL-35) and micro RNA-21(miR-21) in septic patients and to explore the role of their mutual regulation in sepsis .Methods The serum was collected from the patients with sepsis and healthy controls .The expressions of serum IL-35 and miR-21 were detected by ELISA and real-time PCR ,and their correlation was analyzed ;mice were intraperito-neally injected by lipopolysaccharide(LPS) to establish the sepsis model ,then the mice were given anti-IL-35 p35 antibody ,IgG ,sepsis miR-21 mimics(agomir-21) and sepsis miR-21 negative control(agomir-21 NC) by in-traperitoneal injection ,after that ,the serum miR-21 expression level in each group was detected by real-time PCR and IL-35 ,TNF-αand IL-6 levels were detected by ELISA .Results The expression levels of serum IL-35 and miR-21 in sepsis patients were significantly increased compared with the healthy control group ,the difference was statistical significance (P<0 .05) ,moreover their expression levels showed the positive correla-tion (P<0 .05);after septic mice antagonizing to IL-35 ,the serum miR-21 expression was significantly in-creased ,meanwhile the expressions of inflammatory factor TNF-α and IL-6 were increased obviously ,the difference was statistical significance (P<0 .05) .After treating the septic mice by agomir-21 ,the serum IL-35 level was decreased significantly ,while the expression of inflammatory factors TNF-αand IL-6 were increased significantly ,the difference was statistical significance(P<0 .05) .Conclusion Serum IL35 and miR-21 in the patients with sepsis are jointly participate in the septic inflammatory response by mutual regulation .