Establishment and application evaluation of the ELISA method for anti-carbonic anhydrase Ⅱ antibody / 国际检验医学杂志

ABSTRACT

Objective To establish an ELISA detection method for human serum carbonic anhydrase(CA)Ⅱ antibody,and to evaluate the level of serum CA Ⅱ antibody in patients with hypertensive nephropathy, chronic glomerulonephritis,type 2 diabetic nephropathy and healthy people.Methods To establish the ELISA method using CA Ⅱ,anti-CA Ⅱ monoclonal antibody and enzyme labeled secondary antibody,the evaluation of the degree of precision and sensitivity,and stability and anti-interference performance of the ELISA were made.Results The detection accuracy of serum CA Ⅱ antibody ELISA was 6.0%,the precision between the batches was 8.6%,the sensitivity was 0.032,and with favorable anti-interference performance and stability. The level of serum anti-CA Ⅱ antibody was significantly higher in patients with chronic glomerulonephritis and 2-type diabetic nephropathy compared with the normal control(P<0.05).There was no significant differ-ence in serum CAⅡ antibody level between hypertensive nephropathy group and healthy control group(P>0.05).Conclusion It is feasible to establish a serum CAⅡ antibody ELISA method to meet the needs of clini-cal test.CAⅡ antibody may be used as autoantibody to participate in the development of chronic glomerulone-phritis and type 2 diabetic nephropathy.