Analysis of the changes of Th17,Th22,Treg cells and related cytokines in peripheral blood of patients with cervical cancer / 国际检验医学杂志

ABSTRACT

Objective To investigate the changes of helper T cell(Th)17,Th22,regalatory T cell regula (Treg)and related cytokines in peripheral blood of patients with cervical cancer.Methods 95 patients with cervical cancer were enrolled in the study from the hospital from January 2015 to December 2016 as the obser-vation group,meanwhile 61 patients with cervical intraepithelial neoplasia(CIN)treated in the hospital were enrolled in the study as the CIN group,and 56 healthy people who underwent healthy assessment were en-rolled as the healthy group.Peripheral blood was extracted from those people,from which peripheral blood mononuclear cells were isolated,then the percentages of Th17,Th22 and Treg cells were measured,and the se-rum levels of IL-17,IL-21 and IL-22 were measured by using enzyme-linked immunosorbent assay.Results The percentages of Th17,Th22 and Treg cells in the observation group were higher than those in the CIN group and the healthy group;the percentages of Th17,Th22 and Treg cells in the CIN group were higher than those in the healthy group,and the differences were statistically significant(P<0.05);the serum levels of IL-17,IL-21 and IL-22 in the observation group were higher than those in the CIN group and the healthy group, the serum levels of IL-17,IL-21 and IL-22 in the the CIN group were higher than those in the healthy group, and the differences were statistically significant(P<0.05);the precentages of Th17,Th22 and Treg cells in the Ⅲ - Ⅳ stage group were higher than those in Ⅰ - Ⅱ stage group,and the differences were statistically significant(P<0.05);serum levels of IL-17,IL-21 and IL-22 in the Ⅲ ~ Ⅳ stage group were higher than those in Ⅰ - Ⅱ stage group,and the differences were statistically significant(P< 0.05).Conclusion Peripheral blood Th17,Th22,Treg cells and related cytokines may play an important role in the pathogenesis of cervical cancer.