Effects of miR-138 on invasion of brain glioma cells through targeting Sema4C / 国际肿瘤学杂志

ABSTRACT

Objective To explore the effects and mechanism of microRNA-138 (miR-138) on brain glioma cells invasion and migration.Methods The expression of miR-138 was detected by real time quantitative polymcrase chain reaction (qRT-PCR) in 60 cases of glioma tissues and para-carcinoma tissues,and the relationship between miR-138 expression and glioma grading was analyzed.Human glioma cells line U87 and U251 were transfected with miR-138 mimic and negative control (miR-NC).The expression of miR-138 was detected by qRT-PCR.The migration and invasion abilities were tested by Transwell assay,and the expression of semaphoring 4C (Sema4C) protein was tested by Western blotting.Results The expression level of mniR-138 in glioma tissues (2.46 ± 1.07) was significantly lower than that in normal brain tissues (4.83 ±1.16,t =-11.631,P ＜0.001),and miR-138 expression was negatively correlated with tumor grade (r =-O.563,P =0.001).The expression level of miR-138 in cells was significantly higher after being transfected with miR-138 mimic (U251:3.96 ±0.16;U87:4.43 ±0.96) than miR-NC (U251:2.32 ±0.36;U87:2.58± 0.62,t =7.253,P ＜ 0.001;t =8.872,P ＜ 0.001).The ability of invasion and migration were lower after being transfected with miR-138 mimic (U251:89±9;U87:95 ± 10) than miR-NC (U251:206 ± 15;U87:240 ± 20,t =36.629,P ＜ 0.001;t =35.521,P ＜ 0.001).The expression of Sema4C protein was lower after being transfected with miR-138 mimic (U251:0.41 ± 0.06;U87:0.36-± 0.03) than miR-NC (U251:1.01±0.08;U87:1.03±0.13,t=-32.862,P＜0.001;t=-27.512,P＜0.001).Conclusion The up-regulated expression of miR-138 can suppress glioma cells migration and invasion,which might be related to the negative regulation expression of Sema4C protein.