Overexpression of human telomerase reverse transcriptase inhibits myocardial apoptosis induced by hypoxia/reoxigenation in rats / 基础医学与临床

ABSTRACT

Objective To investigate the effects of human telomerase reverse transcriptase (TERT) on myocardial apoptosis induced by hypoxia/reoxigenation (Hypo/Ro) in rats. Methods Adenovirus mediated transfection of TERT gene into myocardial cells of neonatal rats, lentivirus-mediated siRNA silencing the TERT. To investigate the protective effects of human telomerase reverse transcriptase on myocardial hypoxia/reoxigenation injury in rats. Results The apoptosis rate of Hypo/Ro group was significantly higher than that of the control group. Compared with the Hypo/Ro group, TERT over-expression group significantly reduced the rate of apoptosis, and over-expression of TERT decreased the expression of CytC gene and increased the expression of P21 gene in cardiomyo-cytes (P＜0.05).Conclusions Over expression of telomerase reverse transcriptase inhibits myocardial apoptosis induced by Hypo/Ro. The mechanism may be explained by the regulation to the expression of P21 and CytC genes.