Synergistic antitumor effect of HSP90 inhibitor P7 combined with docetaxel in triple-negative breast cancer / 基础医学与临床

ABSTRACT

Objective To evaluate the effect of docetaxel(DTX)combined with a heptapeptide(LPLTPLP, namely P7)on the human triple-negative breast cancer cell line MDA-MB-231 in vitro.Methods The cell viability was measured by SRB assay followed by evaluation of their combination effect using the isobologram.Flow cytometry was performed to quantify apoptotic cells following treatment of DTX,P7 and DTX combined with P7 and the ex-pressions of the apoptosis-related proteins were determined by Western blot analysis.Results P7 synergized with DTX in cell viability detection.The apoptotic rate was highly increased with increasing Bax /Bcl-2 ratio after MDA-MB-231 cells were exposed to DTX and P 7 combination.Conclusions Combination of P 7 and DTX has synergistic effects on MDA-MB-231 cells characterized by increasing apoptosis activation,in which the expression of pro-apop-totic protein Bax increased with a decline in apoptotic protein Bcl-2.