Primary culture and identification of cells from human aldosterone-producing adenoma / 基础医学与临床

ABSTRACT

Objective To establish the primary culture of aldosterone-producing adenoma cells. Methods The tumor tissue was digested by collagen type I and cultured in complete DMEM/F12 medium. Aldosterone concentra-tion in culture medium was detected by radioimmunoassay. The expression of aldosterone synthase in the culture cells was detected by immunofluorescence. Results Aldosterone-producing adenoma cells grew adherently in a round or approximately round shape. The cells were positively immunostained for aldosterone synthase. The aldoste-rone concentration in the culture medium at the 5th culture day was (30.0±8.9)nmol/L. During the primary cul-ture,aldosterone secretion was the strongest at the first day,and decreased afterwards. It kept stable from day 4 to 11. Conclusions We successfully established the primary culture of aldosterone-producing adenoma cells, which are important for the future studying on the mechanism and function of aldosterone-producing adenoma.