Sympathetic excitation and dendritic cell activation aggravates myocardial injury in LPS-induced rats / 基础医学与临床
Basic & Clinical Medicine
;
(12): 973-977, 2018.
Artigo
em Chinês
| WPRIM
| ID: wpr-694019
ABSTRACT
Objective To investigate the effects of sympathetic excitation and dendritic cell activation on myocar-dial injury in LPS-induced rats. Methods The rats were randomly divided into four groupscontrol group, LPS group ( intraperitoneal injection of LPS 10 mg/kg) ,intervention group ( given beta receptor blocker Ate 5 mg/kg after LPS administration) and DC inhibitor VAG539 intervention group ( VAG539 30 mg/kg gavage twice a day for 2 days after LPS administration) respectively . The powerlab system was used to record the hemodynamic and sympathetic data. The concentration of norepinephrine ( NE) in plasma was measured by high performance liquid chromatography ( HPLC) , and the expression and positive cells of TNF-α and DCs in myocardium were detected by immunohistochemistry. Results Compared with the control group, the plasma NE level significantly increased ( P<0.05) ;The expression of TNF-α and DCs in heart tissue significantly increased ( P<0.05) ;the renal sym-pathetic nerve activity (SNA) significantly increased in the three groups after LPS administration for 24 hours. Compared with the LPS group, the plasma NE level significantly decreased ( P<0.05 ) ; The expression of TNF-α and DCs in heart tissue significantly decreased ( P<0.05) ;the renal SNA significantly decreased after Ate and VAG539 administration(P<0.05).Conclusions Excessive activation of sympathetic nervous system and activation of DC aggravate myocardial injury in LPS-induced rats.
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Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Basic & Clinical Medicine
Ano de publicação:
2018
Tipo de documento:
Artigo
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